원문정보
초록
영어
In this study, we identified DNA profiles of tree related 12 residues found at the crime scene and the suspected 89 trees in the farm by microsatellites analysis of tetranucleotide repeat motif at B4630 and B4637 loci. As a result, we observed 9 kinds of DNA profiles in 12 evidences of the crime scene and 75 kinds of DNA profiles in 89 evidences of the farm. Significantly, evidence No. 2 and No. 8 of crime scene matched with evidence No. 75 of the farm in B4630 176 bp and B4637 323 bp. Evidence No. 4 of crime scene matched with evidence No. 69, 77, 84 of the farm in B4630 176 bp and B4637 327 bp, 335 bp. Evidence No. 5 and No. 11 of crime scene matched with evidence No. 22, 57, 82 of the farm in B4630 150 bp, 158 bp and B4637 323 bp, 327 bp. Evidence No. 9 of crime scene matched with evidence No. 37, 40, 63 of the farm in B4630 146 bp, 150 bp, 158 bp and B4637 323 bp, 335 bp. As a consequence, we observed 1~3 pattern of allele in B4630 loci and 1~2 pattern of allele in B4637 loci of every evidence. And almost every allele showed tetranucleotide repeat motif. In conclusion, the research of many more kinds of microsatellite and multiplex PCR needs in the future and as the result we can prepare legal evidence with speed and accuracy against the future coming soon.
목차
I. 서론
II. 재료 및 방법
1. 식물 감정시료와 전체 DNA의 분리
2. 유전자 증폭
3. DNA 증폭확인 및 염기서열분석
4. 대립유전자 확인
Ⅲ. 결과
1. 나무에서 DNA 분리 및 염기서열분석
2. Pinus densiflora의 B4630과 B4637 좌위에 대한대립유전자 (allele) 분석
Ⅳ. 고찰
Ⅴ. 참고문헌