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논문검색

사례

소나무 절도사건에서 개체식별마커 연구에 의한 감정사례

원문정보

A Case of Identification by Research of Microsatellite Markers in Pine Theft

황인관, 김민희, 김미진, 곽경돈, 이은정, 이양한, 박기원

피인용수 : 0(자료제공 : 네이버학술정보)

초록

영어

In this study, we identified DNA profiles of tree related 12 residues found at the crime scene and the suspected 89 trees in the farm by microsatellites analysis of tetranucleotide repeat motif at B4630 and B4637 loci. As a result, we observed 9 kinds of DNA profiles in 12 evidences of the crime scene and 75 kinds of DNA profiles in 89 evidences of the farm. Significantly, evidence No. 2 and No. 8 of crime scene matched with evidence No. 75 of the farm in B4630 176 bp and B4637 323 bp. Evidence No. 4 of crime scene matched with evidence No. 69, 77, 84 of the farm in B4630 176 bp and B4637 327 bp, 335 bp. Evidence No. 5 and No. 11 of crime scene matched with evidence No. 22, 57, 82 of the farm in B4630 150 bp, 158 bp and B4637 323 bp, 327 bp. Evidence No. 9 of crime scene matched with evidence No. 37, 40, 63 of the farm in B4630 146 bp, 150 bp, 158 bp and B4637 323 bp, 335 bp. As a consequence, we observed 1~3 pattern of allele in B4630 loci and 1~2 pattern of allele in B4637 loci of every evidence. And almost every allele showed tetranucleotide repeat motif. In conclusion, the research of many more kinds of microsatellite and multiplex PCR needs in the future and as the result we can prepare legal evidence with speed and accuracy against the future coming soon.

목차

Abstract
 I. 서론
 II. 재료 및 방법
  1. 식물 감정시료와 전체 DNA의 분리
  2. 유전자 증폭
  3. DNA 증폭확인 및 염기서열분석
  4. 대립유전자 확인
 Ⅲ. 결과
  1. 나무에서 DNA 분리 및 염기서열분석
  2. Pinus densiflora의 B4630과 B4637 좌위에 대한대립유전자 (allele) 분석
 Ⅳ. 고찰
 Ⅴ. 참고문헌

저자정보

  • 황인관 In Kwan Hwang. 국립과학수사연구원 유전자감식센터
  • 김민희 Min-Hee Kim. 국립과학수사연구원 유전자감식센터
  • 김미진 Mijin Kim. 국립과학수사연구원 유전자감식센터
  • 곽경돈 Kyoung-Don Kwak. 국립과학수사연구원 유전자감식센터
  • 이은정 Eun-Jung Lee. 국립과학수사연구원 유전자감식센터
  • 이양한 Yang-Han Lee. 국립과학수사연구원 유전자감식센터
  • 박기원 Ki-Won Park. 국립과학수사연구원 유전자감식센터

참고문헌

자료제공 : 네이버학술정보

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