earticle

영어

The efficacy of forensic genotyping as an investigative tool has led to the continual expansion of national DNA databases as genotype profiles are added at an ever-increasing rate. To decrease the risk of false matches in the growing DNA databases, ENFSI and EDNAP recommended an extension of the European Standard Set (ESS) of loci. Following these recommendations, STR multiplexes have been developed by several commercial companies with the extended ESS of loci. AmplSTRⓇ NGM SElectTM PCR Amplification kit (Applied Biosystems) is the next generation kit contains amplify 10 loci plus the 5 new markers (D10S1248, D1S1656, D22S1045, D2S441, D12S391) recommended by ENFSI and EDNAP plus the highly discriminating SE33. With the aim of increasing STR markers of the missing children DNA databases in Korea we introduced new multiplex STR kit, AmplSTRⓇ NGM SElectTM kit (Applied Biosystems). In this study, we present the results of forensic validation studies with the NGM SElectTM kit including following aspects: reproducibility, sensitivity, performance on forensic samples, mixture sample analysis and kinship analysis. The NGM SElectTM kit showed comparable performances to the previously developed STR kits. NGM SElectTM kit showed improved performances, especially in regard to its sensitivity. Kinship analysis and forensic casework sample analysis result showed the NGM SElectTM kit are very informative for forensic purposes and the 6 additional loci provides a good tool for deficiency paternity cases.