earticle

영어

We have analysed the expression and function of cell cycle and cell death regulator Aven in porcine oocytes. Aven is expressed in a wide variety of adult tissue and cell lines, and there is increased evidence of that its role in cell cycle regulation. The mechanism by which anti apoptotic activity of Aven is poorly understood. Although Aven ortholog have been reported in other mammalian species, no Aven gene has been reported in porcine oocytes. Here we shed light on this issue for the first time in porcine oocytes demonstrating that Aven can be potential oocyte quality marker and a novel inhibitor of caspase activation. Our results revealed there is gradual increase in Aven m-RNA expression upto 4-cell stage and then decreased drastically.This result shows Aven is a maternal gene and is regulated dynamically. In addition. Aven protein localisation,caspase-3 activation and mitochondrial distribution were studied by immunofloroscence. Induction of apoptosis by etoposide drug treatment resulted in changes in Aven localisation within COC, corresponding to caspase-3 activation and altered mitochondrial distribution. These results could be an excellent example for studying the function of Aven and identifying cellular factors that influence its activity,revealing information that may be relevant to human disease.