원문정보
초록
영어
The cryopreservation of unfertilized oocytes is valuable for both livestock and human.
However, freezing procedure to oocyte induce many kinds damages such as cytoskeleton
disorganization, and chromosome and DNA abnormalities. Especially, during mammalian
oocyte meiotic maturation, a process called oocyte polarization occurs, which
leads to a unique asymmetric division. Actin play key roles during oocyte polarization
experiences several steps including spindle migration, spindle anchoring and cortical
reorganization. However, how chilling stress affect to actin nucleation in oocytes is
poorly studied. In this study, we investigated expressions and feature of Arp2/3 (actin
activators), WAVE2 and JMY (nucleation promoting factors) in MІІ stage oocytes after
different freezing procedure in oocytes. Results indicated that survival, IVF, developmental
rates of vitrified-thawed oocytes were significantly higher than those of slow
freezed oocytes. Especially, expressions of Arp2/3, WAVE2 and JMY in vitrified-thawed
oocytes were similar with normal oocytes. However, expression of actin nucleation related
genes in slow freezed group was significantly higher or lower than normal
oocytes. Our results may suggested that slow freezing procedure to oocyte induced
abnormal actin neucleation results in low survival and developmental rates.