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As a more convinced technique, somatic cell nuclear transfer (SCNT) has been successfully applied in the production of transgenic farm animals although the cloning efficiency is still low. One of the significant advantages of transgenesis is in rapid genetic improvement of interested traits such as growth rate, muscle growth, disease resistance. In this talk, I focused on the improved modifications of bovine SCNT protocols and its application in the production of fat-1 transgenic dairy and beef cattle. We generated mfat-1 transgenic cattle expressed a Caenorhabditis elegans gene, mfat-1, encoding an n-3 fatty acid desaturase. Fatty acids analysis of tissue and milk derived from transgenic positive cow showed that all of the examined n-3 polyunsaturated fatty acids (PUAFs) were greatly increased and simultaneously the n-6 PUAFs decreased. A significantly reduction of n-6/n-3 ratios (p<0.05) in both tissue and milk were observed. Similar results were obtained in the transgenic beef cattle. Thus, the results indicate that fat-1 transgenic cattle can produce meat and milk enriched in n-3 fatty acids, which probably become an efficient and economical approach to meet the increasing demand for n-3 PUAFs.