earticle

영어

Porcine pluripotent stem cells have important in modeling embryonic development and disease processes in biomedical research, especially to transplantation medicine, immunology and circulatory system. However, despite years of effort, the pluripotency machinery of porcine PSCs has not been demonstrated clearly. Porcine induced plu-ripotent stem cells (piPSCs) were generated using combination of six human tran-scriptional factors under stem cell culture condition. To confirm dependency of these piPSCs, they were separately cultured in stem cell media supplemented with i) 20 ng/ml LIF, ii) 20 ng/ml bFGF, iii) 20 ng/ml BMP4, iv) 20 ng/ml LIF and Su5402, or v) 20 ng/ml bFGF and Jak inhibitor I, for 48 h, respectively. Upon bFGF treated groups, colonies show signs of differentiation with dispersing colony morphology and losing alkaline phosphatase activity. In contrast, colonies with tightly expanded and strong AP activity are shown in LIF or BMP4 treated groups. Western blotting shows phospho- Stat3 and phospho-Smad1/5 are significantly suppressed by Jak inhibitor I. We con-clude that piPSCs generated by six human pluripotent factors, are dependent on LIF or BMP4 to sustain self-renewal and pluripotency.