원문정보
초록
영어
The objective of this study was to evaluate antioxidant effect and tyrosinase inhibitory activity of methanol extracts from Hypsizygus marmoreus. The Hypsizygus marmoreus was divided into two parts (pileus and stipe) and extracted with methanol. Total polyphenolics and flavonoids in the methanol extracts were measured by spectrophotometric methods and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities have been determined for antioxidant activities. The total polyphenolics and flavonoids contents of methanol extract of the pileus were higher than methanol extract of the stipes. The total polyphenolics contents in methanol extracts of the pileus and stipes were 8.7 ug/mg and 5.6 ug/mg, respectively. The total flavonoids contents in methanol extracts of the pileus and stipes were 2.8 ug/mg and 1.4 ug/mg, respectively. The tyrosinase inhibitory activity was proportional to concentration of methanol extract. The tyrosinase inhibitory activity of the methanol extract (200 mg/ml) of pileus (66.9%) and stipe (57.97%) was lower than those of positive control 2% arbutin. The DPPH radical scavenging activity of the methanol extract (20 mg/ml) of pileus and stipes was 52.55% and 30.35%, respectively. Moreover, the effects of methanol extarcts on cell proliferation of B16BL6 mouse melanoma cells were investigated using WST-1 assay (4-[3-(4-iodophenyl)-2- (4-nitrophenyl)-2H-5-tetrazolio]-1,3- benzene disulphonate) and B16BL6 mouse melanoma cells treated with methanol extract of 200-2,000 ug/ml were higher proliferation rate than those of 0.04% adenosine.
목차
I. 서론
II. 재료 및 방법
갈색 느티만가닥버섯 메탄올 추출물의 제조
총 폴리페놀 함량 측정
총 플라보노이드 함량 측정
Tyrosinase 저해 활성
DPPH 라디칼소거 활성
B16BL6 세포 배양
세포 생존율 측정
통계처리
결과 및 고찰
갈색 느티만가닥버섯 메탄올 추출물의 총 폴리페놀및 플라보노이드 함량
갈색 느티만가닥버섯 메탄올 추출물의 tyrosinase 저해 활성
갈색 느티만가닥버섯 메탄올 추출물의 DPPH 라디칼소거 활성
갈색 느티만가닥버섯 메탄올 추출물이 B16BL6 mouse melanoma cell에 미치는 영향
적요
감사의 말씀
참고문헌