earticle

영어

The present study demonstrates green approach for the production of spherical-shaped silver nanoparticles synthesized and stabilized using a bacterium, Eschericia coli. Aqueous solutions of Ag+ionsofsilverweretreatedwiththe supernatant of E. coli for the formation of Ag nanoparticles (AgNPs). The nanometallic dispersions were characterized by surface plasmon absorbance measuring at 420 nm. Transmission electron microscopy and particle analyser showed the formation of nanoparticles in the range of 15～20 nm. X-ray diffraction (XRD) analysis of the AgNPs confirmed the formation of metallic silver. Further analysis carried out by Fourier Transform Infrared Spectroscopy (FTIR), provides evidence for the presence of proteins as possible biomolecules responsible for the reduction and capping agent which helps in increasing the stability of the synthesized AgNPs. The biological activities of the synthesized particles were confirmed based on toxicity assays and lactate dehydrogenase activity (LDH) in A549 cells. The toxicity assay suggests that cell death and leakage of LDH is dose and time dependent. Further, we evaluated the impact of AgNPs on the level of phosphorylation using various kinases such as EGFR, Akt, and Erk. Further, cytotoxicity, inflammation, and developmental toxicity studies shows significant effect in mice. To understand the underlying mechanism of AgNPs on the cellular responses, we showed that AgNPs could inhibit the phosphorylation of EGFR/Akt/Erk. Together, our results indicate that AgNPs can act as an anti-proliferative molecule by targeting the inactivation of EGFR/ Akt signaling pathways.