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논문검색

Poster Session Ⅰ : 배양, 분류, 유전자원, 유전, 육종

Development of SCAR Marker associated Thermophilic Strains of Pleurotus eryngii by Bulked Segregant Analysis

초록

영어

We have used bulked segregant analysis to screen the strain-specific DNA marker associated thermophilic strain of Pleurotus eryngii. Bulked genomic DNAs of Pleurotus eryngii were amplified by randomly amplified polymorphic DNA (RAPD) using OP-A, OP-B, OP-L, OP-P, OP-R and OP-S primers to screen the strain-specific DNA marker. A unique DNA fragment of 550 bp was amplified with OP-S07 primer from the thermophilic strain and sequenced. A sequence characterized amplified region (SCAR) marker was designed on the basis of the determined sequence and named as OP-S07-1. The PCR analysis with the OP-S07-1 primer showed that this SCAR marker clearly distinguish the thermophilic strains from the control strains.

저자정보

  • Hae Soo Kim Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology, Jinju 660-758, Korea
  • Su Cheol Kim Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology, Jinju 660-758, Korea
  • A Rim Nam Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology, Jinju 660-758, Korea
  • So Yeon Park Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology, Jinju 660-758, Korea
  • Hong Chul Kim Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology, Jinju 660-758, Korea
  • Hyun Sook Kwon Swine Science and Technology Center, Gyeongnam National University of Science and Technology, Jinju, 660-758, Korea
  • Jae-San Ryu Eco-friendliness Research Department , Gyeongnam-do Agricultural Research and Extension Services, Jinju 660-360, Korea
  • Soo Jeong Cho Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology, Jinju 660-758, Korea

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