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Original Article

Efficient Plant Regeneration from Petal Segment of Standard-Type Chrysanthemum

초록

영어

An efficient plant regeneration protocol is developed for a standard-type chrysanthemum. When petal segments derived from flower buds (4 or 8cm in diameter) were used as the culture material, the highest shoot regeneration frequency (96%) was obtained on a Murashige and Skoog (MS) medium supplemented with 0.5 mg/L IAA, 2 mg/L BA, 3% sucrose, and a 0.8% agar. Pre-culturing the explants under dark conditions for 14 days produced better results for the shoot regeneration frequency than the explants cultured under a continuous 16 h photoperiod (40 μmolm-2 s -1 ). The shoot regeneration frequency ranged from 19.0% for the Shinmato cultivar to 89.1% for the Baeksun cultivar. Activated charcoal (0.2%) enhanced the root formation of the regenerated shoots in a hormone-free MS medium. The rooted plantlets were acclimatized and successfully established in a greenhouse.

저자정보

  • Mahesh Chhetri Department of Horticultural Science, College of Agriculture and Life Science, Kyungpook National University
  • Su-Min Jeon Department of Horticultural Science, College of Agriculture and Life Science, Kyungpook National University
  • Aung Htay Naing Department of Horticultural Science, College of Agriculture and Life Science, Kyungpook National University
  • Chang Kil Kim Department of Horticultural Science, College of Agriculture and Life Science, Kyungpook National University

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