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느릅나무버섯 자실체의 메탄올과 열수추출물의 항산화, 항염증 및 멜라닌 생성저해 활성

원문정보

Free radical scavenging, anti-inflammatory and melannin synthesis inhibitory activities of Gloeostereum incarnatum

권예주, 김미현, 최재순, 이태수

피인용수 : 0(자료제공 : 네이버학술정보)

초록

영어

Gloeostereum incarnatum is an edible and medicinal mushroom belongs to Family Cyphellaceae of Polyporales, Basidiomycota. The purpose of this study was to investigate the free radical scavenging, anti- inflammatory, and melanin synthesis inhibitory activities of fruiting bodies of Gloeostereum incarnatum. In the free radical scavenging activities, the mushroom extracts showed good 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging and chelating activity on the ferrous ions compared with the positive control, BHT. The mushroom extract suppressed nitric oxide (NO) production in LPS- induced RAW 264.7 macrophage cells in dose dependant manners. Significant reduction of paw edema of rats were observed at 2~6 h after administration with 50 mg/kg of the methanol and hot-water extracts, which were comparable with treatment of 5 mg/kg of indomethacin, the positive control. The melanin synthesis of Melanoma B16/F10 cells treated with 100 μg/mL of the methanol and hot water extracts decreased melanin concentration to 50% and 45% compared with the control, arbutin. Therefore, the experimental results showed that methanol and hot-water extracts of Gloeostereum incarnatum fruiting bodies might be used for good sources of anti-inflammatory, free radical scavenging, and skin whitening agents for human health.

목차

ABSTRACT
 서론
 재료 및 방법
  실험재료
  성분의 추출 및 분리
  총 폴리페놀 함량 측정
  총 플라보노이드 함량 측정
  DPPH 라디칼 소거 활성 측정
  철 이온 제거능 측정
  환원력 활성 측정
  추출물의 세포 독성 측정
  Nitrite (NO) 생성 저해효과 측정
  Carrageenan에 의한 흰쥐 뒷발의 부종유발 및 소염효과의 측정
  티로시나아제 활성 저해능 측정
  Melanoma B16/F10 세포내 멜라닌의 생성억제 측정
  통계 처리
 결과 및 고찰
  총 폴리페놀 및 플라보노이드의 함량
  DPPH 라디칼 소거활성
  철 이온 제거능
  환원력
  추출물의 세포독성
  NO 생성 저해
  Carrageenan에 의한 부종유발 및 항염 효과
  티로시나아제 활성 저해
  Melanoma B16/F10 세포내 멜라닌의 생성 저해
 적요
 감사의 글
 참고문헌

저자정보

  • 권예주 Ye Ju Kwon. 인천논현고등학교
  • 김미현 Mi-Hyeon Kim. 인천논현고등학교
  • 최재순 Jae Soon Choi. 인천논현고등학교
  • 이태수 Tae Soo Lee. 인천대학교 생명과학기술대학, 생명과학부

참고문헌

자료제공 : 네이버학술정보

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