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Purpose: The purpose of this study was to investigate the effect of oral health education on adolescents. Methods:The subjects were middle school students in a health promoting school in Gunpo, Gyeonggi Province. Before giving th students oral health instruction, their modified Patient Hygiene Performance index (PHP index) was measured using disclosing solution. Then, the students were taught the right way to brush teeth (Rolling method) and use oral hygiene devices such as dental floss, tongue cleaner and interdental brush. Their modified PHP index was re-measured three days after and seven days after the education to evaluate the effect and persistency of oral health education. of the total 116 students, 48 students who completely filled out the questionnaire and participated in the whole process, photo shooting and three times of PHP index measurement, were included in the final analysis. Results: The students’ modified PHP index significantly decreased three days after the education. However, the index slightly went back up seven days after the oral health education. Conclusion: Oral health education was effective in reducing dental plaque which remained after brushing. Continuous education about and constant motivation for oral hygiene turned out to be critical to maintain the impact of oral health education. Therefore, it is necessary to develop educational contents which can constantly motivate teenagers.