원문정보
Anti-oxidant and Anti-obese Activities of Turmeric (Curcuma longa L.) Extract in 3T3-L1 Cells
초록
영어
Turmeric (Curcuma longa L.) has been used as a spice such as curries. Although its various biological functions including anti-allergenic, and anti-carcinogenic activities were investigated, the total phenolic content of water and ethanol turmeric extracts and correlation between phenolic content and biological functions including antioxidant and anti-adipogenic activities of turmeric are relatively unknown. Here, we prepared water and ethanol turmeric extracts (W-TE and E-TE). The total phenolic content of W-TE and E-TE were 1.64%, and 9.31%, respectively. To examine the antioxidant activities of W-TE and E-TE, DPPH assay was employed. The E-TE showed strong scavenging activity against DPPH radicals than that of W-TE. The MTT assay was employed to evaluate the cytotoxicity of E-TE on 3T3-L1 cells. The treatment of 10, 25, and 50 μg/ml E-TE for 24 h dose not affect to the 3T3-L1 cell viability. Therefore, the 10 and 50 μg/ml of E-TE were chose further experiment. We also found that the 3T3-L1 adipogenesis was dose-dependently and significantly inhibited by 10, and 50 μg/ml E-TE treatment. The 50 μg/ml of E-TE suppressed 3T3-L1 adipogenesis by 71.53% when compared to adipocytes control. Moreover, the markedly suppressed lipid droplets were observed in E-TE treated 3T3-L1 adipocytes. The evidences suggest that the E-TE has the potential as a functional ingredient in the development of functional foods and nutri-cosmetics on overweight and obesity.
목차
Ⅰ. 서론
Ⅱ. 연구방법
1. 실험재료
2. 울금 추출물의 제조
3. 총 페놀 함량
4. DPPH 라디컬 소거능
5. 울금 추출물의 세포독성
6. 3T3-L1 세포의 배양 및 지방세포분화
7. Oil Red O (ORO) 염색법을 통한 항비만 활성평가
8. 현미경을 통한 지방세포의 수 및 크기 관찰
9. 통계분석
Ⅲ. 연구결과 및 고찰
1. 울금 추출물의 총 페놀 함량
2. 울금 추출물의 항산화 활성
3. 울금 추출물의 세포독성
4. 3T3-L1세포에서 울금 추출물의 adipogenesis 억제활성
5. 지방세포의 수 및 크기 관찰
Ⅳ. 결론
참고문헌