원문정보
Volatile Compounds Analysis of the Extract from Dried Bark of Prunus sargentii and Physiological Activity of the Main Compound, Benzaldehyde
초록
영어
In the present study, we investigated the volatile-flavor compounds in barks of Prunus sargentii and physiological activity of benzaldehyde, main compound of the volatiles. The volatile compounds analyses was performed by gas chromatography with mass selective detector after simultaneous distillation extraction (SDE). As a results, yield of SDE extract was 0.0241%, and fourteen compounds were identified. The principal compounds of the volatiles in SDE extract from barks of P. sargentii are benzaldehyde (34.592%), hexanoic acid (22.863%), hexanal (10.736%), anethole (6.958%), (-)-menthol (5.964%), 2-amylfuran (5.368%). Moreover, piperitone, coumarin, benzyl alcohol, ethylacetate, 1-pentanol, methyl hexanoic acid, 2,4-decadienal and α-isomenthone were identified as small and trace compounds. In antibacterial activity by the disc diffusion assay, benzaldehyde showed antibacterial activity against Staphylococcus aureus , Staphylococcus epidermidis and Pseudomonas aeruginosa . Moreover, benzaldehyde showed dosedependent nitric oxide (NO) production inhibitory effect in lipopolysaccharide (LPS)-stimulated RAW 264.7 cell. As a result, the volatile-flavor compounds in barks of P. sargentii could be applicable to cosmetic and fragrance industry.
목차
Ⅰ. 서론
Ⅱ. 재료 및 방법
1. 재료
2. 사용 균주 및 세포주
3. 향기성분 추출
4. 향기성분의 분석
5. Nitrite Oxide 생성 억제 활성 측정
6. Disc diffusion assay에 의한 항균활성 측정
7. 통계분석
Ⅲ. 연구결과 및 고찰
1. 화피 SDE 추출물의 추출수율
2. 화피 SDE 추출물의 방향성분 분석
3. Benzaldehyde의 nitric oxide 생성 억제활성
4. Benzaldehyde의 항균활성
Ⅳ. 결론
참고문헌