원문정보
초록
영어
Attention deficit/hyperactivity disorder (ADHD) is a neurobehavioral disorder of cognition. We investigated the effects of treadmill exercise on Purkinje cell and astrocytic reaction in the cerebellum of the ADHD rat. Adult male spontaneously hypertensive rats (SHR) and Wistar-Kyo-to rats (WKYR) weighing 210±10 g were used. The animals were ran-domly divided into four groups (n=15): control group, ADHD group, ADHD and methylphenidate (MPH)-treated group, ADHD and treadmill exercise group. The rats in the MPH-treated group as a positive control received 1 mg/kg MPH orally once a day for 28 consecutive days. The rats in the treadmill exercise group were made to run on a treadmill for 30 min once a day for 28 days. Motor coordination and balance were determined by vertical pole test. Immunohistochemistry for the expres-sion of calbindinD-28 and glial fibrillary acidic protein (GFAP) in the cer-ebellar vermis and Western blot for GFAP, Bax, and Bcl-2 were con-ducted. In the present results, ADHD significantly decreased balance and the number of calbindin-positive cells, while GFAP expression and Bax/Bcl-2 ratio in the cerebellum were significantly increased in the ADHD group compared to the control group (P<0.05, respectively). In contrast, treadmill exercise and MPH alleviated the ADHD-induced the decrease of balance and the number of calbindine-positive cells, and the increase of GFAP expression and Bax/Bcl-2 ratio in the cerebellum (P<0.05, respectively). Therefore, the present results suggested that treadmill exercise might exert ameliorating effect on ADHD through re-duction of Purkinje cell loss and astrocytic reaction in the cerebellum.
목차
INTRODUCTION
MATERIALS AND METHODS
Experimental animals and treatments
Vertical pole test
Treadmill exercise protocol
Tissue preparation
Immunohistochemistry for calbindinD-28k and GFAP
Western blot analysis
Data analysis
RESULTS
Effect of treadmill exercise on balance in the vertical pole test
Effect of treadmill exercise on survival of Purkinje cells in the cerebellum
Effect of treadmill exercise on GFAP protein expression in the cerebellum
Effect of treadmill exercise on Bax and Bcl-2 protein expression in the cerebellums
DISCUSSION
CONFLICT OF INTEREST
ACKNOWLEDGMENTS
REFERENCES