원문정보
In vitro Propagation and Protocorm-like Body Formation of Endangered Species, Dendrobium moniliforme
초록
영어
This experiment was conducted to establish the in vitro propagation of Dendrobium moniliforme through protocorm like body (PLB) induction from the culture of leaf, stem and root explants. Frequency of PLB formation from root explants was higher than those of leaf and stem explants. PLB’s proliferation in MS medium including 1.0 mg/L NAA was better than 1.0 mg/L IBA. Frequency of PLB’s proliferation on medium with various concentrations of BA, Kinetin, Tidiazuron (0. 1.0, 3.0 mg/L) and NAA (0, 1.0 mg/L) was tested. The maximun proliferation of PLB’s was obtained on MS medium supplemented with 3.0 mg/L BA and 1.0 mg/L NAA after 6 weeks of culture. Addition of 200 mg/L activated charcoal (AC) and 30 g/L sucrose was effective on PLB proliferation for D. moniliforme.
목차
서론
재료 및 방법
식물재료 및 기내배양조건
석곡의 종자발아 및 배양부위별 유사원괴체(protocormlike body) 유도
석곡의 PLB 증식에 미치는 식물생장조절물질의 영향
신초의 발달 및 발근에 미치는 활성탄(Activated charcoal)및 sucrose의 영향
통계분석
결과 및 고찰
배양부위 및 식물생장조절물질(auxins)농도에 따른 캘러스 유도
신초의 발달 및 발근에 미치는 AC 및 sucrose의 영향
결론
References