원문정보
초록
영어
Citrus peel is a useful research resource containing important physiological function materials. In this study, anti-inflammatory effects of Citrus peel extracts were identified in LPS-induced macrophage cells. After the pretreatment of lipopolysarcharide (LPS), inhibitory effects of nitric oxide (NO), inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2) and pro-inflammatory cytokines, tumor necrotic factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1 (IL-1), production were measured according to the treatment of citrus peel extracts. Study results showed approximately 40% inhibition of NO production but no concentration-dependent effects when citrus peel extracts were treated. According to the results of inhibitory effects of pro-inflammatory cytokines production, IL-6 had about 44% inhibition of production, TNF-α had about 13% inhibition of production, and IL-1 had no inhibition of production. According to western blot results, iNOS protein’s expression was slightly decreased. Also, COX-2 protein’s expression was observed to be slightly decreased in cells treated with citrus peels as iNOS’s result.
목차
1. Introduction
2. Materials and Methods
2.1. Manufacture of extracts
2.2. Cell culture
2.3. MTT cell viability assay
2.4. NO measuring generation inhibitory activity
2.5. Enzyme linked-immunosorbant assay (ELISA)
2.6. Immunoblot analysis
2.7. Statistical analysis
3. Results
3.1. Effect of citrus peels extracts on cellular cytotoxicity
3.2. Inhibitory effect of citrus peels extracts on LPS-induced NO production
3.3. Inhibitory effect of citrus peels extracts on LPS-induced iNOS and COX-2
3.4. Inhibitory effect of citrus peels extracts on LPS-induced cytokine production
4. Discussion
5. Conclusion
References