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영어

We cloned and sequenced the FSHβ and LHβ subunits from the pituitary of the Chromis notata. The partial-length cDNAs of FSHβ and LHβ were 346 bp and 275 bp, encoding 114 amino acid (aa) and 91 aa, respectively. The deduced amino acid sequences of FSHβ and LHβ were highly homologous (75-93%) to those of other Perciformes; Channa maculata, European seabass, TOreochromis mossambicus and Dicentrarchus labrax. Phylogenetic analysis showed that the deduced FSHβ amino acid sequences is closely related to the rainbow trout and tilapia, and LHβ amino acid sequences were are closely related to the Mozambique tilapia and C. maculata. The FSHβ was present in brain, pituitary, liver, gonad but LHβ mRNA exhibited high abundance in the pituitary gland, as determined by RT-PCR.