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Poster Session Ⅰ : 배양, 분류, 유전자원, 유전, 육종

Development of SCAR marker associated thermophilic strains of Pleurotus eryngii by bulked segregant analysis

원문정보

Yun Jin Cho, Su Cheol Kim, Hye Sung Hwang, Jae-San Ryu, Soo Jeong Cho

한국버섯학회 버섯 제17권 2호 통권 28 2013.11 pp.120-121
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초록

영어

We have used bulked segregant analysis to screen the strain-specific DNA marker associated thermophilic strain of Pleurotus eryngii. Bulked genomic DNAs of Pleurotus eryngii were amplified by randomly amplified polymorphic DNA (RAPD) using OP-A, OP-B, OP-L, OP-P, OP-R and OP-S primers to screen the strain-specific DNA marker. A unique DNA fragment of 500 bp was amplified with OP-A11 primer from the psychrophilic strain and sequenced. A sequence characterized amplified region (SCAR) marker was designed on the basis of the determined sequence and named as OP-A11-1. The PCR analysis with the OP-A11-1 primer showed that this SCAR marker clearly distinguish the psychrophilic strains from the control strains.

저자정보

  • Yun Jin Cho Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology, Jinju 660-758, Korea
  • Su Cheol Kim Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology, Jinju 660-758, Korea
  • Hye Sung Hwang Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology, Jinju 660-758, Korea
  • Jae-San Ryu Eco-friendliness Research Department , Gyeongnam-do Agricultural Research and Extension Services, Jinju 660-360, Korea
  • Soo Jeong Cho Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology, Jinju 660-758, Korea

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자료제공 : 네이버학술정보

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