earticle

영어

Maintaining and preserving mushroom mycelial cultures for ensuring the genetic, morphological, physiological traits and viability over time are essential elements in mushroom production. A number of different mushroom species can be preserved by many means, such as cryopreservation, freeze-drying, and placement in preservation solutions like mineral oil, distilled water, or glycerol. This study tested simple and low-cost preservation methods of the mushroom strains from various species, namely Pleurotus ostreatus, Agaricus bisporus, Cordyceps militaris. Each mycelium was cultured and preserved in different storage temperatures and methods. Temperature were differed at 4, 14, 24, 34°C at agar slants. Different storage methods were cryobial tube with sterile water, cryobial tube + 10% glycerol, agar slant of test tube, and agar slant + mineral oil at 4°C for 2 years. The mushroom cultures were better in the viability and recovery at 4°C than those of preservation at higher temperatures. Result showed that all the mushroom strains tested remained viable without any contamination under all different storage methods over two year duration of the experiment. However, all the strains studied could be best stored in sterile water with 10% glycerol for the viability.