earticle

영어

Since chitosan, a natural and polycationic polysaccharide, is a biocompatible, biodegradable, and low immunogenic properties, it has been extensively investigated in biomedical field such as drug delivery, dene delivery, and tissue engineering [2,3]. Chitosan is known to accelarate drug delivery the mucosal layer without tissue damage [4]. Furthermore, chitosan has been frequently employed for conjugation of bioactive chemicals or formation of polyelectrolyte complexes with anionic molecules since chitosan has cationic amine functional grous [3]. For this study, we synthesized hydrophobically modified chitosan using phenethyl isothiocyanate (ChitoPEITC) for photosensitizer delivery to tumor cells. Chlorin E6 (Ce6) was used as a photosensitizer and Ce6-incorporated ChitoPEITC nanoparticles were prepared by simple mixing of Ce6 and ChitoPEITC. Nanoparticles have small particle sizes less than 300 nm. When Ce6-incorporated nanoparticles were treated to HuCC-T1 cholangiocarcinoma cells, nanoparticles improved Ce6 uptake into tumor cells compared to free Ce6. Furthermore, nanoparticles also enhanced ROS generation in tumor cells. Furthermore, nanoparticles revealed higher phototoxicity against tumor cells than free Ce6. We suggest that ChitoPEITC is a promising vehicles for photosensitizer delivery.