earticle

영어

N-linked Glycosylation is enzymatic process that attaches sugars residue to tripeptide sequence Asparagine-X-Serine /Threonin of nascent polypeptide, where X could be any amino acid except Proline. Extracellular and endomembrane protein are mostly glycosylated by N-linked oligosaccharides in eukaryote. The maturation of N-glycan is modified by glycosidases and glycosyl transferases in the ER and golgi of the secretory pathway. The GNUT is a glycosyltransferases that responsible for the transfer core sugar residues to N-glycans of glycoprotein. To explore the physiological role of alpha GNUT, the mutant gnut1 was isolated in rice. Genetic analysis shows that T-DNA was inserted in the first intron of the GNUT gene. The T-DNA insertion causes loss-of-function mutation of this gene. Biochemical analysis have confirm that sugar residue was absent from core N-glycan in this mutant. The gnut1 shows internodes length retardation in the late vegetative growth. In generative growth, the gnut1 mutant also exhibit short panicles length, grain filling defective and small grains. The result indicates that GNUT is important for normal growth of rice in the generative phase.