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영어

Anoikis is an anchorage-dependent cell death. Anoikis resistance is known to be a crucial step for cancer metastasis. We have previously identified that N-Acetylglucosaminyl transfer ase-V (GnT-V) is a key protein inducing metastasis in human colon cancer cells. And the secretion of galectin-3 binding protein (LGALS3BP) was lowered in GnT-V overexpressing WiDr cells. In this study, we hypothesized that GnT-V has an inhibitory effect on anoikis of cancer cells, while LGALS3BP stimulates the apoptotic process. To prove this, we established the stable transfectants (WiDr:GnT-V), the control cells (WiDr:mock). As a result, GnT-V was observed to mitigate anoikis and promote cell aggregation in soft- agar plate system. Among human colon cancer cells tested, HCT116 cells formed aggregates poorly and thus showed low cell viability. Of note, the secretion of LGALS3BP was higher than any other colon cancer cellline, which is in line with the result that anoikis resistance was observed in LGALS3BP-suppressed WiDr cells. Taken together, these results may suggest that GnT-V induces anoikis resistance of cancer cells either alone or in cooperation with the target molecules, such as LGALS3BP.