earticle

영어

Seaweed extracts have been reported for their diverse biological and pharmacological activities including immunomodulating, anticoagulaning, anticancer activities, etc. Capsosiphon fulvescens (CF) is a green alga that is widely ditributed in the Southern coastal area of Korea. Crude polysaccharides were obtained from the CF collected at a coastal area of Wando, Korea by extraction in acidic condition (0.01 N HCl) and 75% ethanol precipitaion. After removal of alginate by CaCl2 precipitaion, it was futher purified by DEAE-cellulose column chromatography and its chemical components and anticancer activity was determined. Monosaccharide composition analysis by TLC and HPEAC-PAD showed that this polysaccharide consist of Rha, Man and Xyl as the major neutral sugars, with the mole ratios of 4.1:1.0:3.7, respectively, suggesting that it is a mannoxylorhamnan type polysaccharide. This polysaccharide (1 mg/ml) was shown to effectively scavenge nitrite up to 40% at pH 3. The effects of this polysccharide on the cell viability of 4 cancer cell types, human prostate cancer cell (PC-3), human lung cancer cell (A-549), human colon cancer cell (HT-29), and human gastric cancer cell (MK-N-45), were evaluated by MTT assay. It showed most significant cytotoxicity against HT-29 cells among cancer cell lines tested. This polysaccharide significantly induced the cleavage of PARP, caspase 8 and caspase 9, and also enhanced the phosphorylation of p38 in a dose-dependant manner. However, it was shown to reduce the AKT phosphorylation and mitochondrial membrane charge causing mitochondrial dysfunction. Taken collectively, these results demonstated that a watersoluble polysaccharide isolated from the sporophyll of Korean Capsosiphon fulvescens inhibits proliferation of HT-29 cells by activation of apoptotic pathway, suggesting that this polysaccharide can be a good candidate for the development of a potent anti-cancer agent against human colon cancer.