earticle

영어

Hyaluronan (also called hyaluronic acid or hyaluronate) is an anionic, nonsulfated glycosaminoglycan distributed widely throughout connective, epithelial, and neural tissues. Hyaluronan, a core component of the extracellular matrix, comprises a repeating oligosaccharides of N-acetylglucosamine and D-glucuronic acid. The purpose of this study was to investigate the anti-adipogenic effect of low-molecular weight hyaluronan (LMW-HA, 43,500 Da) on 3T3-L1 preadipocyte cells. LMW-HA did not show cytotoxicity on 3T3-L1 preadipocyte cells up to 200 mg/ml. Treatment of 3T3-L1 cells maintained in adipocyte-induction media with 25, 50, 100, and 200 mg/ml of LMW-HA resulted in significant decrease in intracellular lipid drops by 75.5, 65.4, 58.0, and 53.5%, respectively, suggesting that LMW-HA has anti-adipogenic effect on 3T3-L1 adipocyte differentiation. Triglyceride (TG) levels in the cells treated with varying concentrations of LMW-HA (25, 50, 100, 200 μg/ml) were significantly decreased in a dose-dependent manner, with 53% reduction at 200 μg/ml. However, incubation of cells with LWM-HA (25, 50, 100, and 200 mg/ml) significantly reduced the mRNA level of PPAR-γ by 81, 69, 58, and 56%, respectively, and also aP2 by 81, 67, 56, and 55%, respectively, compared to untreated control group. In addition, the protein levels of PPAR-γ, C/EBP-α and aP2 in LMW-HA treated groups also decreased in a dose-dependent manner. PPAR-γ, C/EBP-α and aP2 are known as the major adipocyte-specific genes. Therefore, these results suggested that LMW-HA has anti-obesity effect by inhibiting adipocyte differentiation through down-regulation of PPAR-γ, C/EBP-α and aP2 expression.