earticle

영어

As one of the most important compounds in nature, carbohydrates have attracted much attention in the fields of chemical biology, therapeutics, synthetic chemistry and so on. During the last 15 years, engineered glycosidases, including glycosynthases, thioglycoligases and O-glycoligases, have emerged as very powerful tools to address the synthesis of various functional carbohydrates, such as oligosaccharides, glycolipids and glycoproteins. These mutated glycosidase, whose catalytic residue (nucleophile or general acid/base) is replaced by an inactive residue, loss their hydrolytic activity and show significantly transglycosylation activities. Many elegant cases have been reported so far. Here, the different catalytic mechanisms and advances of engineered glycosidases in the synthesis of carbohydrates were summarized.