원문정보
초록
영어
Plants are considered to represent a promising expression system for production of valuable recombinant immunotherapeutic proteins. Plants as an expression system has several advantages: lack of animal pathogenic contaminants, low cost of production, and ease of agricultural scale-up compared to available systems. We have studied to enhance expression efficiency of immunotherapeutic recombinant protein GA733, colorectal cancer vaccine candidate with fusion of human IgG Fc fragment and KDEL, endoplasmic reticulum retention signal resulting in GA733-FcK. Furthermore, selectively harvesting plant tissues highly expressing GA733-FcK from entire plant biomass is essential to improve final productivity. Thus, in this study, we have established several transgenic plant lines to highly express GA733-FcK and investigated variable expression level of recombinant GA733-FcK proteins along with leaf and stem tissues. The mRNA and protein levels in top, middle, and bottom leaf or stem tissues were observed using real time PCR and immunoblot analyses. In addition, the glycan structures and DNA methylation patterns were studied with respect of aging of plant tissues.