earticle

영어

Free oligosaccharides in human milk (HMO) have multiple biological functions, for example, the growth of beneficial bacteria in the gut and the development of brain. And they also provides important property for the postnatal development of the immune system. Until now, HMO has studied through MALDI-TOF MS and nano-LC/Chip Q-TOF MS for profiling and qualitative analysis. On the other hand, UPLC-Triple quadrupole MS is five fold faster than nano-LC/Chip Q-TOF MS. In this study, we have profiled and quantified free oligosaccharides extracted from HMO. And UPLC-Triple quadrupole MS is well-suited quantitative analysis on HMO. Here, we were comparable to data of HMO using MALDI-TOF, nano-LC/Chip Q-TOF and UPLC-Triple Quadrupole for verifying the suitability of UPLC-Trople Quadrupole MS on HMO analysis. Extraction of free oligosaccharides was performed by following steps including the removal of lipids by Folch method and ethanol protein precipitation. To get rid of αand β isomers, free oligosaccharides were reduced by reductive amination. Extracted free oligosaccharides were further purified by solid phase extraction using a porous graphitized carbon cartridge. Purified and enriched free oligosaccharides were redissolved in water prior to mass spectrometry analysis. First, we obtained that 64 free oligosaccharides from MALDI-TOF MS and 110 free oligosaccharides from nano-LC/Chip Q-TOF MS. Top Fifteen of free oligosaccharides profiled by nano-LC/Chip Q-TOF MS were found also by UPLC-Triple quadrupole MS. This study can be applied as an analytical platform to extract, profile, and quantify free oligosaccharides by UPLC-Triple quadrupole MS in milk and dairy products.