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Session V, 좌장 : 추영국 교수 (원광대학교)

Industrial application of hyperthermophilic L-arabinose isomerase

초록

영어

D-Tagatose, which is an isomer of D-galactose, is a natural ketohexose sweetener and rarely found in nature. Since D-tagatose has been classified as a generally recognized as safe (GRAS), it can be used in confectionary, beverage, health foods, and dietary products as a low-calorie bulk sweetener. Moreover, it can be potentially used for health functional ingredients having anti-hyperglycemic function. Initial quantities of D-tagatose have been produced commercially by a chemical reaction using calcium catalyst at Arla Foods Inc. However, since the chemical process had disadvantages on limited conversion yield and purification performance, several patented L-arabinose isomerase (AIs) have been exploited as potential resources for the commercial galactose-tagatose isomerization process. The industrially enzymatic reaction for the production of D-tagatose should be operated at high temperatures above 55oC to minimize the risk of contamination, to improve the mass transfer, and to lower the viscosity of substrates. Similar to High Fructose Corn Syrup (HFCS) process, higher yields of conversion can be also achieved by increasing the reaction temperature. AI from Thermotoganeapolitana was commercially produced and immobilized as biocatalysts for continuous semi-fluidized reactor system. The isomerization resultants containing 40~45%(w/v) of D-tagatose was purified further by ion-exchange chromatography followed by simulated moving-bed chromatography and crystallization. The final crystalline products consist of more than 99% of D-tagatose and less than 1% of others (sugar, ash, and water). In 2011, CJ Cheiljedang built up a new commercial facility for the commercial production of D-tagatose (1,800MT/yr). Since then, CJ has sold it as a novel food ingredient in South Korea and US. The operational stability and reduced process impurities (waster material) are important key properties for mass production of bulk sugar ingredient. The enzymatic isomerization process should be more unique and practical method for the large scale production of D-tagatose.

목차

1.Education Background
 2. Work Carrier
 3. Research Field
 4. Research Interests
 5. Publication
 Industrial application of hyperthermophilic L-arabinose isomerase

저자정보

  • 김성보 Seong-Bo Kim. CJ 제일제당 선임연구원
  • Sung-JaeYang Sweetener Ingredient Research Center, CJ Cheiljedang Corp, 636 Guro-dong, Guro-gu, Seoul, 152-050, Korea
  • Yond-HoHong Sweetener Ingredient Research Center, CJ Cheiljedang Corp, 636 Guro-dong, Guro-gu, Seoul, 152-050, Korea
  • Dong-Woo Lee Division of Applied Biology and Chemistry, Kyungpook National University, 80 Daehak-ro, Buk-gu, Daegu, 702-701,Korea

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