원문정보
초록
영어
A water-soluble polysaccharide (SPS-CF) was isolated and purified from Korean green alga Maesaengi Capsosiphon fulvescens mainly by dilute acid extraction, ethanol precipitation, and DEAE-cellulose ion exchange chromatography. The results of present study performed for monosaccharide composition of this polysaccharide using HPLC of PMP labelled sugars, HPAEC-PAD, GC-FID and TLC, clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (44–52 mol %) and rhamnose (34–45 mol %), while mannose and galactose are present at much lesser extent or in negligible amount. These extensive monosaccharide analyses and correlation NMR measurements confirmed that it is a sulphated glucuronorhamnoxylan (ulvan) type polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains. The SPS-CF exhibited significant invitro anticoagulant activity by which the activated partial thromboplastin time (aPTT) and thrombin time (TT) were significantly prolonged. Also, Enzyme Linked Immunosorbent Assay showed that the SPS-CF significantly stimulates the release of the pro-inflammatory cytokines, TNF-α and IL-6, in a dose-dependent manner. RT-PCR analysis demonstrated that the SPS-CF also induced a more than two-fold increase in the expression of iNOS and COX-2, responsible for the induction of NO and PGE2, respectively, at 5 μg/ml in RAW264.7 murine macrophages. The results of this study demonstrated that the SPS-CF isolated from Korean C. fulvescens Maesaengi is an ulvan type polysaccharide and can be considered as potential anticoagulant and immunostimulating agent.