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논문검색

Session IV : 신진연구자, 좌장 : 이균오 교수 (경상대학교)

Structural analysis and bioactivities of a polysaccharide isolated from Capsosiphonfulvescence (Masaengi)

초록

영어

A water-soluble polysaccharide (SPS-CF) was isolated and purified from Korean green alga Maesaengi Capsosiphon fulvescens mainly by dilute acid extraction, ethanol precipitation, and DEAE-cellulose ion exchange chromatography. The results of present study performed for monosaccharide composition of this polysaccharide using HPLC of PMP labelled sugars, HPAEC-PAD, GC-FID and TLC, clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (44–52 mol %) and rhamnose (34–45 mol %), while mannose and galactose are present at much lesser extent or in negligible amount. These extensive monosaccharide analyses and correlation NMR measurements confirmed that it is a sulphated glucuronorhamnoxylan (ulvan) type polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains. The SPS-CF exhibited significant invitro anticoagulant activity by which the activated partial thromboplastin time (aPTT) and thrombin time (TT) were significantly prolonged. Also, Enzyme Linked Immunosorbent Assay showed that the SPS-CF significantly stimulates the release of the pro-inflammatory cytokines, TNF-α and IL-6, in a dose-dependent manner. RT-PCR analysis demonstrated that the SPS-CF also induced a more than two-fold increase in the expression of iNOS and COX-2, responsible for the induction of NO and PGE2, respectively, at 5 μg/ml in RAW264.7 murine macrophages. The results of this study demonstrated that the SPS-CF isolated from Korean C. fulvescens Maesaengi is an ulvan type polysaccharide and can be considered as potential anticoagulant and immunostimulating agent.

저자정보

  • Doo Jin Choi Department of Biotechnology and The Research Centre for Biopharmaceutical Lead Molecule (GRRC), TheCatholic University of Korea, Bucheon, Gyeonggi-do 420-743, Republic of Korea
  • Ji Won Choi Department of Biotechnology and The Research Centre for Biopharmaceutical Lead Molecule (GRRC), TheCatholic University of Korea, Bucheon, Gyeonggi-do 420-743, Republic of Korea
  • Jisun Lee Department of Biotechnology and The Research Centre for Biopharmaceutical Lead Molecule (GRRC), TheCatholic University of Korea, Bucheon, Gyeonggi-do 420-743, Republic of Korea
  • Chang Won Lee Department of Biotechnology and The Research Centre for Biopharmaceutical Lead Molecule (GRRC), TheCatholic University of Korea, Bucheon, Gyeonggi-do 420-743, Republic of Korea
  • Seul Lee Department of Biotechnology and The Research Centre for Biopharmaceutical Lead Molecule (GRRC), TheCatholic University of Korea, Bucheon, Gyeonggi-do 420-743, Republic of Korea
  • Andriy Synytsya Department of Carbohydrate Chemistry and Technology, Institute of Chemical Technology in Prague, Technicka 5, 166 28 Prague 6, Czech Republic
  • Yong Il Park Department of Biotechnology and The Research Centre for Biopharmaceutical Lead Molecule (GRRC), TheCatholic University of Korea, Bucheon, Gyeonggi-do 420-743, Republic of Korea

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