원문정보
초록
영어
A carbohydrate chip has been suggested as a powerful tool for robust, rapid, and diverse screening of carbohydrate-protein interaction. Here we proposed a facile, efficient, and cost-effective method whereby diverse carbohydrate types are modified in a single step and directly immobilized onto a glass surface, with retention of functional orientation. We modified various types of carbohydrate by reductive amination, in which reducing sugar groups were coupled with 4-(2-aminoethyl)aniline, which has amine groups at both ends. The modified carbohydrates were attached to amine-reactive NHS-activated glass surface by formation of stable amide bonds. This new method was applied for efficient construction of a carbohydrate microarray platform to analyze carbohydrate-protein interactions. This array prepared using our method can be successfully used for screening the specificity of GBPs (glycan-binding proteins) such as lectins and microbial toxins, and identification of carbohydrate-protein interactions