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Purpose: This study was examined the characteristics of bacteria isolated from the dental stone that is made ??inthe dental laboratory. Methods: 104 dental stones samples were collected from the 4 dental laboratory. Characteristics of bacteria wereinvestigated by microorganism isolation culture method using a Blood Tryptic Soy Agar(TSA) medium. Results: The detected various bacteria was confirmed as pathogenic bacteria, non-pathogenic bacteria and naturalbacteria. The isolated bacterial number was confirmed 2.9 103CFU and maxium bacterial number of 3.0 104CFU. Conclusion: Therefore, infection prevention education is required, it must be to live up the hand-washing andwear protective clothing to protect themselves when working in a dental laboratory.