원문정보
초록
영어
To profile the proteome in porcine plasma, blood samples were collected from adult male barrows and those plasma were retrieved. For the depletion or pre-fractionation of high-abundance proteins, plasma samples were treated with commercial kits. Then, protein profiling was initiated using one and two-dimensional electrophoresis. Proteins were spotted and then identified by MALDI-TOF-TOF and LC-MS-MS. In the results, more than forty six proteins were identified and the reference map was constructed. The pre-treatment for the removal of high-abundance proteins caused the changes in 2-DE images and some of the proteins were newly uncovered after the most of high abundant proteins were removed. However, it is expected for further steps necessary to identify more low-abundance proteins that may contain potential bio-markers.
목차
INTRODUCTION
MATERIALS AND METHODS
Animals
Protein Preparation for 2-DE
2-DE and Image Analysis
Protein Identification
RESULTS AND DISCUSSION
Proteomic Profiling: Forty Six Proteins Identified (MALDI-ToF-ToF, 19 Proteins; LC-MS-MS, 37 Proteins)
Abundant Protein Removals 2-DE Proteome Image of Plasma Partially Depleted with Abundant Proteins
REFERENCES