원문정보
초록
영어
N-terminal kinase-like (NTKL) protein was initially identified as a protein binding to protein kinase B (PKB, also known as Akt). Though NTKL-BP1 (NTKL-binding protein 1) has been identified as an NTKL binding protein, its functions related to binding have not yet been elucidated. Here, a new alternative spliced variant of NTKL and its association with integrin β1 is described, in addition to the kinase activity of NTKL and its substrate candidates. Although the phosphorylation of the candidates must be further confirmed using other experimental methods, the observation that NTKL can phosphorylate ROCK1, DYRK3, and MST1 indicates that NTKL may act as a signaling protein to regulate actin assembly, cell migration, cell growth, and to facilitate differentiation and development in an integrin- associated manner.
목차
1. Introduction
2. Experimental Section
2.1. Cell Culture and Antibodies
2.2. Isolation of FAC
2.3. Hybridoma Production
2.4. Immunoscreening
2.5. Construction of Plasmids
2.6. Immunofluorescence Microscopy
2.7. Co-immunoprecipitation and Immunokinase Assay
2.8. Poison Primer Technique
2.9. Northern Hybridization
2.10. Protoarray Kinase Substrate Identification
3. Results and Discussion
3.1. Immunoscreening and Tissue Distribution of a New NTKL Variant
3.2. NTKL is Associated with Integrin β1
3.3. NTKL has Kinase Activity
4. Conclusions
References