원문정보
The Effects of Er-miao-San Extract on Cell Proliferation in Normal Human Dermal Fibroblasts
초록
영어
This study set out to investigate the effects of Er-Miao-San extracts to which the same amount of Phellodendri cortex (P. cortex ) and Atractylodis japonica (A. japonica ) was added on the proliferation in HDFs and also the synergistic effects of Er-Miao-San prescription. For that purpose, the study looked into DPPH radical scavenging, WST assay, cell proliferation rate, cell doubling time, and cell migration. The DPPH radical scavenging results show that Er-Miao-San extracts recorded the most excellent anti-oxidant effects compared with hot water extracts when being treated with high concentration 2 mg/mL in 70% ethanol. In the WST assay experiment, it was confirmed that Er-Miao-San extracts had the highest cell viability compared with P. cortex and A. japonica and increased the cell proliferation rate depending on the concentration. As for the cell doubling time of Er- Miao-San extracts, P. cortex and A. japonica , Er-Miao-San extracts was the shortest at 12 hours and 44 minutes at 500 μg/ mL. In the cell migration experiment, Er-Miao-San extracts proved faster cell proliferation than P. cortex and A. japonica . From these results, Er-Miao-San extracts recorded the highest effects of anti-oxidant scavenging activity, cell viability, cell proliferation rate, and cell migration compared with P. cortex and A. japonica , showed adequate potential for application in cosmetic production and related industries as well as a functional material.
목차
Ⅰ. 서론
Ⅱ. 연구방법
1. 시료 및 추출
2. DPPH radical 소거능 측정
3. 세포 배양
4. 세포 생존율 측정
5. 세포 증식도 측정
6. Doubling time 측정
7. 세포 유주 실험
Ⅲ. 연구결과 및 고찰
1. 추출조건에 따른 DPPH radical 소거능
2. 추출물의 세포 생존율 비교
3. 추출물 별 세포 증식 비교
4. 추출물 별 doubling time 비교
5. 추출물 별 세포 유주 비교
Ⅳ. 결론
참고문헌