상백피 추출물의 생물전환과 티로시나제 저해 효과의 비교


Inhibition of Tyrosinase by Bio-conversioned Morus alba Extract

김은주, 문지선, 최태부

피인용수 : 0(자료제공 : 네이버학술정보)



Skin lightening is one of the most important effect of functional cosmetics, especially for the asian women. For this reason, many kinds of inhibitors of melanin synthesis by melanocyte have been developed since a decade ago. Although many kinds of melanogenesis inhibitors such as arbutin, ascorbic acid, extract of Licorice root or Morus alba and niacinamide were recommended as the standard component of the skin whitening cosmetics by Korean Food and Drug Administration, they are not always satisfactory to the many users. Mulberroside A, a main component of extract of Morus alba balk, can be converted into an aglycan named oxyresveratrol having a strong antioxidant and anti-melanogenesis effect using β-glucosidase. However, the glucose hydrolysis process using a soluable β-glucosidase is not suitable to convert Mulberrosie A into oxyresveratrol in a large scale procss because of the low reaction rate and high price. In this work, we developed a bioconversion process of oxyresveratrol production from Morus alba extract using enzyme named Accellerase®, which is an immobilized enzyme on the solid particle, and compared the tyrosinase inhibition effect of the extracts of before and after the bioconversion process. It was found that the inhibition effect of Morus alba extract to the mushroom tyrosinase was greatly increased by the bioconversion process and this effect was synergized when the extract was used with arbutin together.


 Ⅰ. 서론
 Ⅱ. 연구방법
  1. 시약
  2. 세포 배양
  3. 상백피 시료의 제조
  4. 상백피 생물전환
  5. HPLC을 이용한 상백피 추출물 성분분석
  6. Polyphenol 함량 측정
  7. Flavonoid 함량 측정
  8. DPPH radical 소거능 측정
  9. Tyrosinase 저해능 측정
  10. Melanin 생성 저해능 측정
  11. 자료분석 및 통계적 유의성 검정
 Ⅲ. 연구결과 및 고찰
  1. 상백피 추출물의 생물전환
  2. 생물전환 후 항산화활성의 변화
  3. 생물전환 상백피 추출물의 티로시나제 저해
  4. Arbutin과 oxyresveratrol의 티로시나제 저해 상승효과
  5. 멜라닌 생성 저해능 측정
 Ⅳ. 결론


  • 김은주 Eun-Joo Kim. 열린사이버대학교 뷰티디자인학과
  • 문지선 Ji-Sun Moon. 건국대학교 생물공학과
  • 최태부 Tae-Boo Choe. 건국대학교 생물공학과


자료제공 : 네이버학술정보

    함께 이용한 논문

      ※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.

      0개의 논문이 장바구니에 담겼습니다.