원문정보
Effects of the Complex Containing Centella asiatica - and Folic Acid-Fermented Extracts, Acetyl Glutamine, and Nicotinic Acid Adenine Dinucleotide Phosphate on the Inhibition of Senescence and Melanogenesis, Promotion of Collagen Expression, Cellular Regeneration, and Keratinocyte Differentiation, and Anti-inflammation
초록
영어
In this study, we investigated effects of the complex material containing Centella asiatica (C. asiatica )- and folic acidfermented extracts, acetyl glutamine, and nicotinic acid adenine dinucleotide phosphate (NAADP) on the improvement of skin condition and skin cell functions. We confirmed cellular effects of fermented extracts of C. asiatica and folic acid using microorganism mixture (CFFE) and acetyl glutamine- and NAADP-containing CFFE (FAN) on cell viability, reactive oxygen species (ROS) generation, cellular senescence, DNA repair, transcriptional activity of NF-κB, regulation of gene expression, cellular regeneration and migration, cell differentiation, and melanin contents in human dermal fibroblasts (HDFs), HaCaT human keratinocytes, B16F10 mouse melanocytes, and NIH-3T3 mouse embryo fibroblasts. CFFE and FAN showed significant protection effects against cellular damage caused by ultraviolet B (UVB) irradiation or ROS generation. The expression levels of Col1A1 was up-regulated and of MMP1 was down-regulated by treatment of CFFE and FAN in UVB-exposed HDFs. UVB-induced NF-κB activation was significantly suppressed by treatment of CFFE and FAN in NIH-3T3 cells. Also the treatment of CFFE and FAN promoted the differentiation on HaCaT cells. The CFFE and FAN suppressed production of melanin in B16F10 cells by inhibition of expression of MITF and TYR. Especially, it was observed that acetyl glutamine and NAADP increase overall effects of CFFE in earlier time points, implicating that these components initiate and boost cellular signals and positive functions mediated by CFFE in the skin. Taken together, these findings suggest that CFFE and FAN might have excellent efects of the anti-aging, reduction of wrinkle and pigmentation , promotion of cellular regeneration, improvement of epidermal barrier quality, and anti-inflammation on the skin cell.
목차
Ⅰ. 서론
Ⅱ. 연구방법
1. 원료 제조
2. 피부 세포 배양
3. 세포 생존율 측정
4. ROS 변화량 측정
5. DNA 복구 능력 측정
6. 세포노화 측정
7. qRT-PCR을 통한 유전자 발현 확인
8. NF-κB luciferase assay를 통한 NF-κB 전사 활성 변화 측정
9. 세포재생 및 세포이동 능력 측정
10. Western blot을 활용한 단백질 발현 변화 측정
11. 멜라닌 생성량 측정
Ⅲ. 연구결과
1. 세포 생존률에 미치는 영향
2. 세포노화에 미치는 영향
3. 진피섬유아세포의 생리활성에 미치는 영향
4. 염증반응 억제 효과
5. 각질형성세포의 생리활성에 미치는 영향
6. 멜라닌형성세포의 생리활성에 미치는 영향
Ⅳ. 고찰
Ⅴ. 결론
참고문헌