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Antibodies are used for preventing several infectious diseases in food animals. Though, antibiotics can deal with infection however, their widespread usage may cause developments of resistance and can also get transferred to humans through animal products. Therefore, production of antibodies against infectious agents in the egg yolk could be an interesting alternative. The present study was conducted with a focus on producing the antibody against Escherichia coli E68 and H28 in egg yolk of laying hens, which are believed to be a cause for diarrhea and beriberi in piglets. The result received from the experiment were promising and have shown efficiency of antibody production in the range of 2-7.3% in the groups which are infected with E68 only and the one which was infected with both strains. The outcome of present research has revealed the potentiality of egg yolk in production of antibody for laying hens, could open a new approach for production of antibodies to manage diarrhea and beriberi.