원문정보
초록
영어
Fibrinosis, platelet aggregation and thrombosis were the leading hurdles in the patients alleged from myocardial infarction and stroke. Various nutritional factors were influencing the production of multifunctional recombinant staphylokinase variant (Msak – RGD – Hirulog) from salt inducible E.coli GJ1158, which was constructed in our lab. One-factor-at-a-time method was used to investigate the constituents like carbon and nitrogen sources for the best medium composition. Initial optimization was carried out by using L27 - orthogonal array method. The significance of each factor with respect to MRH production was identified by Taguchi (Dextrose Mono Hydrate (DMH), Na2HPO4, KH2PO4, TMM, NH4Cl, Yeast extract and 1M MgSO4) and the specific fibrinolytic activity was increased 1.34 folds (20,058 to 22,763 U/mL). The outcome of taguchi results indicates the dextrose mono hydrate, yeast extract, Na2HPO4 and KH2PO4 are influencing in the production of MRH. Final optimization was carried out using response surface methodology (Central Composite Rotatable Design) and the activity of MRH was significantly enchanced to 1.17 folds (22,763 to 26,758 U/mL). For the first time, cost effective media constituents for enhanced production (960 mg/L) of multi-functional MRH and its activity (26,758 U/mL) was studied.
목차
1. Introduction
2. Materials and methods
2.1. Medium components
2.2. Media preparation and culture conditions
2.3. Optimization of components by using one factor-at-a-time
2.4. Optimization using L27 - Orthogonal array
2.5. Fibrinolytic assay
2.6. Optimization of components of the selected medium by RSM
3. Results & Discussion
3.1. One factor-at-a-time method
3.2. Optimization using L27-orthogonal array
3.3 Optimization of concentrations of the selected medium components by RSM
4. Conclusion
Acknowledgements
References