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Purpose: The purpose of this study was to investigate laughter index, self esteem, subjective happiness, quality of life in nursing college students. Method: The data were collected from 267 nursing college students by means of structured questionnaire. The data were analyzed using descriptive analysis, Pearson correlation analysis by SPSS version 18. Results: The measuring of respondents' laughter index revealed they scored an average of 88(±10.07) of the full score 120. The measuring of respondents' self esteem showed they marked an average of 30.72(±2.64) of the full score 50. The measuring of respondents' subjective happiness showed they marked an average of 15.09(±2.82) of the full score 20. The measuring of respondents' quality of life showed they marked an average of 132(±19.04) of the full score 235. The laughter index related to subjective happiness and quality of life in nursing college students were significantly correlated. Conclusion: This study showed that laughter index in nursing college students were correlated positively subjective happiness and quality of life. Further research is regarded as necessary to evaluate and to compare effects of laughter with physical health status.