원문정보
Comparison of Growth Inhibitory Effects on Cancer Cells of Saponin and Fucoidan Treated with Recombinant Thermophilic Xylose Isomerase
초록
영어
The gene encoding for xylose isomerase from the thermophilic bacterium Thermotoga maritima was cloned and recombinantly expressed in E. coli cells. Optimal activity was shown at 90oC and pH 8.0. Treatment of saponin by recombinant xylose isomerase increased the growth inhibitory effect against human gastric cancer (AGS) cells and human colon cancer (HT-29) cells. On the other hand, treatment of fucoidan by the enzyme could not change the growth inhibitory effect against the same cancer cells. One μg/ml of enzyme-treated saponin exhibited the same or higher growth inhibitory effect against both cancer cells compared with 100 μg/ml of enzymeuntreated saponin. These results would be useful in the development of functional food or drug.
목차
1. 서론
2. 재료 및 방법
2.1. DNA, 시약 및 배지
2.2. 유전자 확보 및 클로닝
2.3. 재조합 xylose isomerase의 발현과 정제
2.4. SDS-PAGE
2.5. 효소의 활성측정
2.6. 재조합 xylose isomerae 처리 전후의 푸코이단과 사포닌의 암세포 생육제해 활성 측정
3. 결과 및 고찰
3.1. Thermotoga maritima의 xylose isomerase 유전자 클로닝
3.2. 발현과 정제
3.3. 온도와 pH에 따른 효소활성과 안정성
3.4. 재조합 xylose isomerae 처리 전후의 푸코이단과 사포닌의 암세포 생육제해 활성
4. 요약
REFERENCES