원문정보
A Study on Anti-oxidant Activity and Anti-inflammatory Action of Sea Buckthorn Seed Extract
초록
영어
This study aimed to observe the effects of Sea Buckthorn seed extracts (SEB) on anti-oxidation and antiinflammation. SEB were found to strongly depress ROS production depending on concentration, and 46.5% anti-oxidation effects were found in the top concentration of 100 μg/ mL. Histamine and arachidonic acid release were measured to observe the effects of SEB on anti-inflammation. SEB inhibited histamine release and arachidonic acid release on dose dependent manner. Based on the results above, the conclusion is drawn that SEB have the potential to be used as natural materials that are effective for anti-oxidation and antiinflammation.
목차
1. 서론
2. 재료 및 방법
2.1. 시약
2.2. 실험 재료
2.3. 세포배양
2.4. MTT를 이용한 세포독성 측정
2.5. Reactive oxygen species (ROS) 측정
2.6. Histamine release 측정
2.7. Arachidonic acid 활성 측정
2.8. 자료분석 및 통계적 검정
3. 결과 및 고찰
3.1. 세포독성 측정
3.2. RAW 264.7 세포에서 reactive oxygen species (ROS)소거 활성
3.3. Histamine release 활성
3.4. Arachidonic acid(AA) release 활성
4. 결론
REFERENCES
