원문정보
Bioethanol Production from Eucheuma spinosum using Various Yeasts
초록
영어
Ethanol fermentations were performed using separate hydrolysis and fermentation (SHF) processes with monosaccharides from pretreated seaweed, Eucheuma spinosum as the biomass. The pretreatment was carried out with 11% (w/v) seaweed slurry and 150 mM H2SO4 at 121oC for 40 min. Enzyme hydrolysis after H2SO4 pretreatment was performed with Celluclast 1.5 L at 45oC for 24 h. Five % active charcoal were added to hydrolysate to removed 5-hydroxy methylfurfural. Ethanol fermentation with 11% (w/v) seaweed hydrolysate was performed for 72~96 h using Kluyvermyces marxianus, Pichia stipits, Saccharomyces cervisiae and Candida tropicalis. Ethanol concentration was reached to 18 g/L by K. marxianus, 16 g/L by P. stipitis, 15 g/L by S. cerevisiae and 10 g/L by C. tropicalis, respectively. The ethanol yield from total monosugar was obtained 0.50 and ethanol productivity was obtained 0.38 g/L/h by K. marxianus.
목차
1. 서론
2. 재료 및 방법
2.1. 재료
2.2. 산 촉매 열 가수분해 및 효소당화
2.3. 활성탄을 이용한 발효물질 제거
2.4. 발효균주
2.5. 분리당화발효 (SHF)
2.6. 분석방법
3. 결과 및 고찰
3.1. E. spinosum의 구성성분
3.2. 효모 4종을 이용한 에탄올 발효
4. 결론
감사
REFERENCES