원문정보
The Extract from Lysimachia foenum-graecum Induces Apoptosis in MCF-7 Breast Cancer Cells
초록
영어
The extract from Lysimachia foenum-graecum (LFE) has been known to possess various instructive characters including anti-oxidant, anti-obesity, fungicidal activities. However, the accurate mechanism of those effects of LFE is not well known. In that respect, we evaluated the apoptotic effect and anti-cancer efficacy of extracts of LFE in MCF-7 breast cancer cells. In this study, we hypothesized that LFE may exert cancer cell apoptosis through regulating p53 and mitochondria-mediated apoptotic proteins. And this substance can generate ROS to cause free radical-induced apoptosis. Accordingly, the generation of ROS by LFE triggers the activation of p53 which are accompanied by pro-apoptotic protein activation and suppression of pro-survival proteins. We determined with MTT assay, flow cytometry for detection of intracellular ROS and Annexin V-PI staining, Western blotting. Consequently, our researches demonstrated that the treatment of LFE to breast cancer cells resulted in an activation of p53, Puma, Bax, cleaved-PARP and an inhibition of Bcl-2 expressions.
목차
1. 서론
2. 재료 및 방법
2.1. 실험재료
2.2. 세포 배양
2.3. MTT assay에 의한 세포생존율 측정
2.4. Fluorescence-Activated Cell Sorting (FACS)에 의한 apoptosis 관찰
2.5. FACS 및 Fluorescence microscope를 이용한 세포 내 ROS 측정
2.6. Western blotting
2.7. 통계처리
3. 결과 및 고찰
3.1. LFE에 의한 MCF-7 세포 증식 억제 및 apoptosis 유도 효과
3.2. LFE에 의한 MCF-7세포에서의 ROS 생성 분석
3.3. LFE 처리에 따른 p53의 활성과 apoptosis 관련 단백질의 조절
3.4. p53의 저해 상태 및 항산화 조건에서 LFE의 처리에 따른 신호분자들의 관계 확인
4. 결론
감사의 글
REFERENCES