원문정보
초록
영어
Marine fungi are the potential and promising sources for biologically active secondary metabolite productions. Secondary metabolites are the chemical compounds that are produced during the stationary phase of the organism. Many years of study revealed that fungi are excellent sources for novel bioactive secondary metabolites. In the present study fungi were isolated from marine water samples and serial dilution method was performed to isolate single colonies. Later microscopic and macroscopic observations were done to identify the organism. Organisms are inoculated into medium for production of secondary metabolites. One week later ethyl acetate is added to the broth in 1:1 ratio and kept for incubation in shaker. Solvent is separated by separating funnel and solvent is extracted by rotavapour to collect the crude extract. Crude extract is dissolved in DMSO and further tested for biological activities. The Fungi was identified as Fusarium species and its secondary metabolites showed potential antimicrobial activity. The present work was attempted to isolate organisms which are capable of producing efficient antibacterial and antifungal compounds. Further we identified the organism as Fusarium sp. The future scope of this work is to isolate these biologically active compounds to use in pharmaceutical applications.
목차
1. Introduction
2. Materials and Methods
2.1. Collection of Sample
2.2. Isolation of Fungi from Soil using Soil Dilution Method
2.3 Microscopic Observation:
2.4. Culturing of the Isolated Fungi
2.5. Extraction of Secondary Metabolites
2.6. Anti-Microbial Activity of the Crude Extract
2.7. Purification by TLC
3. Results
3.1. Amount of Secondary Metabolite Extracted per Batch
3.2. Table 1. Antibacterial Activity of the Crude Extract of Fusarium sps..:
3.3. Separation of Compounds, Chromatography (TLC) by Thin Layer
4. Discussion
5. Conclusion.
References