원문정보
초록
영어
To find the new raw material having skin care activity, P. oleracea of organic native plant was extracted and concentrated with solvent extracting method. As a cosmetic active material, it is to develop an active raw molecule for discovering new skin pathway such as anti-aging, whitening and wrinkle skin improvement. The skin irritating evaluations (in-vitro) were performed to confirm the skin safety of its extract. Both free radical scavenging activity and collagen biosynthesis activity for skin anti-aging activity were evaluated with control sample (pure ascorbic acid). Melanin inhibition activity (in-vitro) as a skin lightning effect, to find the skin whitening was tested to use its material. As an application study, two high functional emulsion lotion having almost natural sources containing P. oleracea extract were formulated without any preservatives and totally evaluated the skin improvement effect (whitening, anti-aging activity and fine wrinkle diminish effect, human volunteer test, in-vivo). Therefore, the organic P. oleracea extract can contribute to the development of new natural active ingredients for advanced skin care cosmetic.
목차
1. Introduction
2. Materials and Methods
2.1. Materials
2.1. Equipment
2.2. Preparation of Extract
2.3. Free Radical Scavenging Effect
2.4. Determination of Collagen Biosynthesis Effect
2.5. Tyrosinase Inhibitive Activity
2.6. Measurement of Melanin Contents in B16 Melanoma
2.7. Human Volunteer Evaluation (in-vivo)
3. Result and Discussions
3.1. High Concentrated Extract of P. oleracea and Determination of Key Ingredients:
3.2. Free Radical Scavenging Effect
3.3. Collagen Synthesis Activity (in-vitro)
3.4. Melanin Inhibitive Test (in-vitro)
3.5. Inhibition of Melanin Formation in B16 Melanoma Cell (in-vitro)
3.6. Skin Whitening and Fine Wrinkle Diminishing Activity (in-vivo)
3.7. Development of Formulation Containing Plant Origin
4. Conclusions
Acknowledgements
References