earticle

영어

The polymerase chain reaction (PCR) is a method utilized in most of the experiments handling genetic materials. PCR amplifies the target genetic material that is to be analyzed. This paper demonstrates the accuracy of the temperature measured by the thermistor. Since the thermistor is attached to the bottom of the micro PCR chip, there becomes a gap between the chamber and the thermistor. This experiment measures the temperature difference due to the gap. Micro PCR chip has a chamber made of double-sided tape and OHP film integrated upon a PCB substrate printed with a heater pattern. The thermistor to measure the chamber temperature is attached to the bottom of the substrate. In the experiment, a thermo-couple was inserted into the chamber directly to measure the actual temperature. The results showed no significant difference between the actual chamber temperature measured by the thermo-couple and the temperature measured by the thermistor attached at the bottom of the substrate.