원문정보
Whitening and Anti-aging Activities of Soluble Silkworm Gland Hydrolysate
초록
영어
Silkworm-derived silk materials which contain 45 to 55% protein (18 amino acids, including 8 essential amino acids) have free radical-scavenging activities. In the present study, we investigated the whitening and anti-aging effects of silkworm gland under various conditions with B16F1 melanoma cells and human dermal fibroblasts. To evaluate the toxicity of soluble gland hydrolysate (SGH), mouse-derived B16F1 melanoma cells were treated with 0.2~5 mg/mL, cytotoxicity was not observed at all concentrations. The tyrosinase and elastase activities were significantly decreased as dosage levels of SGH increased. In addition, cell death was decreased by treated with SGH and antioxidative activity was the most effective in the SGH treatment. These findings suggest that SGH may be used as a potential functional biomaterial in having the skin whitening effect and anti-aging activity by inhibition of tyrosinase and elastase activities.
목차
1. 서론
2. 재료 및 방법
2.1. 세포 배양
2.2. 누에 실샘 가수분해물의 제조
2.3. 세포 생존율 분석
2.4. Tyrosinase 활성 분석
2.5. Elastase 활성 분석
2.6. 활성산소종 활성 분석
2.7. DPPH radical 소거 활성 분석
2.8. 통계 분석
3. 결과 및 고찰
3.1. 세포독성
3.2. Tyrosinase 활성 저해
3.3. Elastase 활성 저해
3.4. 활성산소종 억제
3.5. DPPH radical 소거 활성
4. 결론
REFERENCES