원문정보
Production and Characterization of β-Glucan Type Oligomer Produced with Enzymatic Hydrolysis of Capsosiphon fulvescens
초록
영어
β-Glucan type oligomers which have angiotensin I converting enzyme (ACE) inhibitory activity were isolated and characterized from Capsosiphon fulvescens. After C. fulvescens was hydrolysis with Alcalase at 50oC, supernatant was harvested and separated with ultrafiltration membrane (MWCO 2 kDa). Oligomers which were less than 2 kDa of molecular weight were harvested for characterization. The nutrient composition of Alcalase hydrolysate was 89.9% carbohydrate, 4.2% protein and 5.9% sulfate. After ultrafiltration, the nutrient composition of oligomers was changed to 99.88% carbohydrate, 0.07% protein and 0.05% sulfate. The carbohydrate composition of oligomer was glucose (97.2%) and mannose (1.5%). The ACE inhibitory activities of Alcalase hydrolysate and oligomer were 72.1% and 82%, respectively. The molecular weight of oligomer was about 1 kDa. The oligomer was analyzed with FT-IR, 1H-NMR and methylation. The oligomers were β-1,3-glucans with β-(1,3)-linked glucose units.
목차
1. 서론
2. 재료 및 방법
2.1. 실험 재료
2.2. 효소 추출물 제조
2.3. ACE 저해 활성 측정
2.4. 한외여과막 시스템을 이용한 분리
2.5. HPLC에 의한 분자량 측정
2.6. 화학적 조성 분석
2.7. 구성당 성분분석
2.8. FT-IR 및 NMR 분석
2.9. Methylation-acetylation 분석
3. 결과 및 고찰
3.1. Alcalase 추출물의 ACE 저해활성
3.2. 한외여과막로 분리한 분획물의 ACE 저해활성
3.3. 올리고머의 분자량 측정
3.4. 화학적 조성 및 구성당 성분분석
3.5. 분획의 구조 분석
4. 결론
REFERENCES