연자육 추출물의 멜라닌 합성 저해효과


The Inhibitory Effects of Nelumbo nucifera Gaertner Extract on Melanogenesis

이준영, 임경란, 정택규, 윤경섭

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In order to develop new skin whitening agents, we prepared the CH2Cl2 layer (NGC) and BuOH layer (NGB) of 75% EtOH extract of the Nelumbinis nucifera Gaertner. We measured their tyrosinase inhibitory activity in vitro and melanin synthesis inhibitory activity in B16-F1 melanoma cells. They did not show inhibitory activity against mushroom tyrosinase but showed melanin synthesis inhibitory activity in a dose-dependent manner. In a melanin synthesis inhibition assay, NGC and NGB suppressed melanin production up to 52% and 46% at a concentration of 100 μg/mL, respectively. To elucidate the mechanism of the inhibitory effects of NGC and NGB on melanogenesis, we measured the expression of melanogenesis-related proteins by western blot assay. As a result, NGC suppressed the expression of tyrosinase, tyrosinase related protein 1 (TRP-1), tyrosinase related protein 2 (TRP-2), phosphorylated cAMP responsive element binding (p-CREB) protein, and microphthalmia associated transcription factor (MITF). And NGB inhibited the protein expression of tyrosinase and MITF, but had no significant effect on TRP-1, TRP-2, and p-CREB expression. Moreover, NGB increased the expression of phosphorylated extracellular signal- regulated kinase (p-ERK). In addition, we examined the inhibitory effect on the glycosylation of tyrosinase. As a result, NGC and NGB inhibited the activity of α-glucosidase in vitro and the glycosylation of tyrosinase in B16-F1 melanoma cells. From these results, we concluded that NGC and NGB could be used as active ingredients for skin whitening.


 1. 서론
 2. 재료 및 방법
  2.1. 실험재료, 시약 및 기기
  2.2. 연자육 추출물 및 분획물의 제조
  2.3. Tyrosinase 저해활성 측정
  2.4. 세포주 및 세포배양
  2.5. 세포 생존력 시험
  2.6. 멜라닌 함량 측정
  2.7. 단백질 발현 조사 (Western Blot 분석)
  2.8. α-Glucosidase 저해활성 측정
  2.9. N-Glycosylation 억제효과 측정
  2.10. 통계처리
 3. 결과 및 고찰
  3.1. 세포독성
  3.2. 멜라닌 합성 억제효과
  3.3. Tyrosinase/TRP-1/TRP-2 단백질 발현 저해효과
  3.4. cAMP / PKA 경로에 관련된 신호전달 단백질 발현에 미치는 효과
  3.5. ERK pathway activation에 미치는 효과
  3.6. α-Glucosidase 억제효과
  3.7. N-Glycosylation 저해효과
 4. 결론


  • 이준영 Jun Young Lee. (주)사임당화장품 기술연구소
  • 임경란 Kyung Ran Im. (주)사임당화장품 기술연구소
  • 정택규 Taek Kyu Jung. (주)사임당화장품 기술연구소
  • 윤경섭 Kyung-Sup Yoon. (주)사임당화장품 기술연구소


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