원문정보
The Inhibitory Effects of Nelumbo nucifera Gaertner Extract on Melanogenesis
초록
영어
In order to develop new skin whitening agents, we prepared the CH2Cl2 layer (NGC) and BuOH layer (NGB) of 75% EtOH extract of the Nelumbinis nucifera Gaertner. We measured their tyrosinase inhibitory activity in vitro and melanin synthesis inhibitory activity in B16-F1 melanoma cells. They did not show inhibitory activity against mushroom tyrosinase but showed melanin synthesis inhibitory activity in a dose-dependent manner. In a melanin synthesis inhibition assay, NGC and NGB suppressed melanin production up to 52% and 46% at a concentration of 100 μg/mL, respectively. To elucidate the mechanism of the inhibitory effects of NGC and NGB on melanogenesis, we measured the expression of melanogenesis-related proteins by western blot assay. As a result, NGC suppressed the expression of tyrosinase, tyrosinase related protein 1 (TRP-1), tyrosinase related protein 2 (TRP-2), phosphorylated cAMP responsive element binding (p-CREB) protein, and microphthalmia associated transcription factor (MITF). And NGB inhibited the protein expression of tyrosinase and MITF, but had no significant effect on TRP-1, TRP-2, and p-CREB expression. Moreover, NGB increased the expression of phosphorylated extracellular signal- regulated kinase (p-ERK). In addition, we examined the inhibitory effect on the glycosylation of tyrosinase. As a result, NGC and NGB inhibited the activity of α-glucosidase in vitro and the glycosylation of tyrosinase in B16-F1 melanoma cells. From these results, we concluded that NGC and NGB could be used as active ingredients for skin whitening.
목차
1. 서론
2. 재료 및 방법
2.1. 실험재료, 시약 및 기기
2.2. 연자육 추출물 및 분획물의 제조
2.3. Tyrosinase 저해활성 측정
2.4. 세포주 및 세포배양
2.5. 세포 생존력 시험
2.6. 멜라닌 함량 측정
2.7. 단백질 발현 조사 (Western Blot 분석)
2.8. α-Glucosidase 저해활성 측정
2.9. N-Glycosylation 억제효과 측정
2.10. 통계처리
3. 결과 및 고찰
3.1. 세포독성
3.2. 멜라닌 합성 억제효과
3.3. Tyrosinase/TRP-1/TRP-2 단백질 발현 저해효과
3.4. cAMP / PKA 경로에 관련된 신호전달 단백질 발현에 미치는 효과
3.5. ERK pathway activation에 미치는 효과
3.6. α-Glucosidase 억제효과
3.7. N-Glycosylation 저해효과
4. 결론
감사
REFERENCES